解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (4 ): 576-579.doi: 10.3969/j.issn.0529-1356.2013.04.025

• 技术方法 • 上一篇    下一篇

小鼠肾脏纤维化Masson染色方法的改进

魏胜男 曾翔俊 李汇华*   

  1. 首都医科大学基础医学院病理学与病理生理学系， 北京 100069
  • 收稿日期:2012-08-10 修回日期:2013-01-05 出版日期:2013-08-06 发布日期:2013-09-04
  • 通讯作者: 曾翔俊 E-mail:megan_zeng@163.com
  • 基金资助:

    国家自然科学基金重点资助项目

Improvement of Masson staining in renal fibrosis of mice

WEI Sheng-nan ZENG Xiang-jun LI Hui-hua*   

  1. Department of Pathology and Pathophysiology, Capital Medical University, Beijing 100069, China
  • Received:2012-08-10 Revised:2013-01-05 Online:2013-08-06 Published:2013-09-04

摘要：

目的 改进Masson三色染色法，以获得较好显示肾脏纤维化的染色方法。方法 用Bouin液补充固定组织切片；用-20℃储存的乙醇∶乙酸（2∶1）溶液处理组织；用亮绿代替苯胺蓝；减少亮绿的染色时间。结果 明确区分纤维化肾间质和未纤维化的肾间质，胶原纤维呈绿色（用亮绿复染），
胞质和肌纤维呈红色。结论 改进后的Masson方法可以将纤维化和非纤维化的肾脏组织进行明显的区分，显微镜下结构显示清晰，着色均匀，取得了较为满意的显色对比效果。

关键词: 肾脏, 纤维化, Masson 染色法, 小鼠

Abstract:

Objective To get an improved Masson 3 staining method for staining renal fibrosis. Methods Bouin‘s fluid was used to re-fix the paraffin fixed sections. Ethanol∶acetic acid（2∶1) which was stored at -20℃ was used to treat the sections;
Brilliant green was used to substitute aniline blue. Duration of brilliant green staining was decreased. Results Renal interstitium of fibrosis and non-fibrosis were distinguished. Collagen fibers appeared green (counter staining by brilliant green) and cytoplasm
and muscle fibers were red. Conclusion In improved Masson 3 staining, tissues of fibrosis and non-fibrosis are distinguished by brilliant green, the staining results are stable and uniform. The method demonstrates a satisfied compared staining result.

Key words: Kidney, Fibrosis, Masson staining method, Mouse